MLL-AF4 gene fusions in normal newborns.

نویسندگان

  • M H Kim-Rouille
  • A MacGregor
  • L M Wiedemann
  • M F Greaves
  • C Navarrete
چکیده

1. Uckun FM, Herman-Hatten K, Crotty ML, Sensel MG, Sather HN, Tuel-Ahlgren L, Sarquis MB, Bostrom B, Nachman JB, Steinherz PG, Gaynon PS, Heerema N: Clinical significance of MLL-AF4 fusion transcript expression in the absence of a cytogenetically detectable t(4;11)(q21;q23) chromosomal translocation. Blood 92:810, 1998 2. Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156, 1987 3. Gale KB, Ford AM, Repp R, Borkhardt A, Keller C, Eden OB, Greaves MF: Backtracking leukemia to birth: Identification of clonotypic gene fusion sequences in neonatal blood spots. Proc Natl Acad Sci USA 94:13950, 1997 4. Chen CS, Sorensen PH, Domer PH, Reaman GH, Korsmeyer SJ, Heerema NA, Hammond GD, Kersey JH: Molecular rearrangements on chromosome 11q23 predominate in infant acute lymphoblastic leukemia and are associated with specific biologic variables and poor outcome. Blood 81:2386, 1993 5. Downing JR, Head DR, Raimondi SC, Carroll AJ, Curcio-Brint AM, Motroni TA, Hulshof MG, Pullen DJ, Domer PH: The der(11)encoded MLL/AF-4 fusion transcript is consistently detected in t(4; 11)(q21;q23)-containing acute lymphoblastic leukemia. Blood 83:330, 1994

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Identification of Genes Transcriptionally Responsive to the Loss of MLL Fusions in MLL-Rearranged Acute Lymphoblastic Leukemia

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Trithorax and polycomb cooperation in MLL fusion acute leukemia.

G enetic alterations of the mixed lineage leukemia 1 gene (MLL1, here referred to as MLL) located on the long arm of chromosome 11 (11q23) are found in pediatric (particularly infant) and about 5-10% of adult de novo and therapy-related acute lymphoblastic leukemias (ALL) or acute myeloblastic leukemia (AML); these types of leukemia are often characterized by early relapse. 1,2 Over 50 differen...

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The MLL fusion gene, MLL-AF4, regulates cyclin-dependent kinase inhibitor CDKN1B (p27kip1) expression.

MLL, involved in many chromosomal translocations associated with acute myeloid and lymphoid leukemia, has >50 known partner genes with which it is able to form in-frame fusions. Characterizing important downstream target genes of MLL and of MLL fusion proteins may provide rational therapeutic strategies for the treatment of MLL-associated leukemia. We explored downstream target genes of the mos...

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AF4 encodes a ubiquitous protein that in both native and MLL-AF4 fusion types localizes to subnuclear compartments.

Acute leukemia with t(4;11)(q21,q23) translocation results from the in-frame fusion of the MLL to the AF4/FEL gene. In previous studies, we and others demonstrated that AF4 transcripts are present in a variety of hematopoietic and nonhematopoietic human cells. To further study the wild-type and leukemia fusion AF4, we used glutathione S-transferase (GST)-fusion proteins as immunogens to produce...

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عنوان ژورنال:
  • Blood

دوره 93 3  شماره 

صفحات  -

تاریخ انتشار 1999